ohsu

segundo dia lab

construccion de un plasmido
digerirlo con enzimas
electroforesis

fun+fun+fun
electro

judin ninguna reservacion. hotel benson entonces. ohsu. primer dia de tramites y seminarios con superlunch.

FLIGHT American - AA 908 Mon 24 NOV 2003
Departs: 10:55 PM Ministro Pistarini (EZE) Buenos Aires
Arrives: 05:39 AM Miami Intl (MIA)
Status: Confirmed for Economy class
Conf Nbr for American - EDYPTR
Seats: 38J
Service: 777 - Boeing 777 Journey Time 8:44 Dinner, Breakfast

FLIGHT Delta - DL 1682 Tue 25 NOV 2003
Departs: 12:25 PM Miami Intl (MIA)
Arrives: 3:00 PM Cincinnati No Kentucky Intl Arpt (CVG)
Status: Confirmed for Thrift class
Conf Nbr for Delta - 3DH1RF
Seats: 15D
Service: Arrival Terminal 3 M80 - Douglas MD-80 Journey Time 2:35

FLIGHT Delta - DL 1983 Tue 25 NOV 2003
Departs: 4:50 PM Cincinnati No Kentucky Intl Arpt (CVG)
Arrives: 6:44 PM Portland Intl Arpt (PDX)
Status: Confirmed for Thrift class
Conf Nbr for Delta - 3DH1RF
Service: Departure Terminal 3 763 - Boeing 767 Journey Time 4:54 Food to buy

I am making your reservations to travel to Oregon the last week of November 2003. Is Monday - November 24th alright with you for a departure date? I will make the reservation for departure out of EZE in Buenos Aires, Argentina.

I will also need to know your full name as it appears on your passport.

Thank you.
Nancy McDuffee

Paula,
Thank you for this update - OHSU will be able to provide the medical insurance after your first full month, so I hope the cost of the insurance you need to start with will not be too great. We look forward to your arrival. Judi

y hoy... me dieron la visa.
:)

Dear Paula,

I just heard for Michael Conn that you were offered a position in the Fogarty Program at OHSU and have accepted. Congratulations. I look forward to meeting you and working with you. I hope that the process of obtaining a visa for you will go smoothly. Based on recent experiences, I estimate that it will take several months to confirm everything. Probably an accurate time for you to begin here will be early next year, hopefully by early January. As the time gets closer, I would be happy to help you find housing in Portland, and to assist in any other way. It also would be prudent to develop a schedule of reading to get you up to speed on IGF research in general and on gene regulation by hormones in particular. When you are ready, I can send a reading list, and probably can forward many of the articles as PDF files.

Best regards,

Peter

Dra. Moyano,

Your host laboratory is located on the main campus of the Oregon Health and Science University (Dr. Peter Rotwein, Molecular Medicine Dept). The main campus is approximately 20 kilometers East of the Primate Center (which is part of the OHSU West Campus complex).

Nancy Clark, Dr. Rotwein's administrative manager, may be able to answer questions regarding your work in this lab. Her e-mail address is: clarkn@ohsu.edu. I believe she will be forwarding information to you regarding the on-going research in the lab.

The letter transmitted to you by Dr. Conn, referred to housing available near the Primate Center location only. Because you will be working in a laboratory on the main OHSU campus, I have made tentative arrangements for you to stay at a Bed and Breakfast (Hedlund's Bed and Breakfast) which is within walking distance of OHSU main campus. Their address is:
3412 SW 13th Avenue
Portland, OR 97239
Telephone number: 503-223-6617

Once your travel arrangements have been finalized, we can confirm with Ms. Hedlund, the exact nights you would be staying at her home. This temporary residence will allow you time to look for an apartment to rent.

I am sending you, by Federal Express, a DRAFT of the Exchange Visitor visa document (DS-2019), for your review. If there are no additions or corrections, please let me know, and I will Federal Express the original document to you, for presentation at the US Consulate.

If I can be of further help, please contact me.

Respectfully -
Judith Connor
ONPRC - Personnel Coordinator

please see attached letter.



P. Michael Conn, Ph.D.
Associate Director and Senior Scientist - -ONPRC
Special Assistant to the President and Professor of Physiology and Pharmacology
and of Cell and Developmental Biology - -OHSU
505 NW 185th Avenue
Beaverton, OR 97006
PHN (503) 690-5297
FAX (503) 690-5569

Paula,

Here is a better overview. I have also forwarded it to Sergio Ojeda.

-Peter

Hormonal Control of IGF-I Gene Expression

Peptide growth factors regulate cell division, intermediary metabolism, and differentiation by activating specific cell-surface receptors, and play essential roles in the growth and development of organisms as diverse as flies, worms, frogs, and humans. Our laboratory studies the regulation and actions of the insulin-like growth factors (IGFs), peptides critical for normal embryonic and post-natal growth and for aspects of tissue differentiation and specialization in mammals and other vertebrate species. One of our major interests has been in understanding basic mechanisms responsible for regulating IGF-I gene expression. For the past several years our specific focus has been on hormonal signaling pathways that control IGF-I gene transcription. We have learned that growth hormone (GH) stimulates IGF-I gene expression through the phosphorylation and activation of the transcription factor Stat5b (1), and have recently identified the DNA sequences responsible for mediating this hormonal response (2). We also have shown that the second messenger cAMP and cAMP-dependent protein kinase (PKA) stimulate IGF-I transcription through a novel pathway mediated by the transcription factor, C/EBP delta (3, 4). In bone cells, PKA induces translocation of C/EBP delta from cytoplasm to nucleus by a post-translational mechanism that does not involve phosphorylation of the transcription factor (3, 4). Other groups have demonstrated that the hormone estradiol activates IGF-I gene expression in the endometrium of the uterus (5), although the molecular mechanisms have not been established, and no conventional estrogen-response elements have been identified within the IGF-I gene. As locally produced IGF-I has been postulated to mediated the mitotic actions of estradiol in the endometrium, and has been implicated in estradiol-responsive neoplasms in this tissue (5, 6), understanding the fundamental mechanisms by which estrogen regulates IGF-I gene expression has potential significance for both normal human physiology and disease.

We propose to elucidate the signaling pathways and biochemical steps by which estrogen induces IGF-I gene expression in the uterus. Our laboratory has the expertise to evaluate IGF-I gene regulation in response to hormones in both in vitro and in vivo model systems, as outlined above and in the cited references. Dr. Moyano has expertise in reproductive endocrinology and physiology. In my laboratory, she will first learn the basic approaches to studying IGF-I gene expression, and will then apply them to analysis of control of IGF-I transcription by estradiol and potentially by other hormones and growth factors within the uterus. The general experimental approach will follow the steps that we have used to assess hormonal control of IGF-I gene expression in other tissue types.

References

1. Woelfle J, Billiard J, Rotwein P (2003). Acute control of insulin-like growth factor-I gene transcription by growth hormone through Stat5b. J Biol Chem 278:22696-22702

2. Joachim J, Chia DJ, Rotwein P (2003). Mechanisms of growth hormone action: identification of conserved Stat5 binding sites that mediate GH-induced insulin-like growth factor-I gene activation. Manuscript submitted

3. Billiard J, Umayahara Y, Wiren K, Centrella M, McCarthy TL, Rotwein P (2001). Regulated nuclear * cytoplasmic localization of CCAAT/Enhancer Binding Protein d in Osteoblasts. J Biol Chem 276:15354-15361

4. Billiard, J., Grewal, S.S., Lukaesko, L., Stork, P.J.S., Rotwein, P. (2001). Hormonal control of insulin-like growth factor I gene transcription in human osteoblasts: dual actions of cAMP-dependent protein kinase on CCAAT/enhancer binding protein delta. J Biol Chem 276:31238-31246

5. Murphy LJ, Murphy LC, Friesen HC (1987). A role for the insulin-like growth factors as estromedins in the rat uterus. Trans Assoc Am Phys 99:204-214

6. Rutanen E-M, Nyman T, Lehtovirta P, Ammala M, Pekonen F (1994). Suppressed expression of insulin-like growth factor binding protein-1 mRNA in the endometrium: a molecular mechanism associating endometrial cancer with its risk factors. In J Cancer 59:307-312